Functional genomic screening allows for the elucidation of the mechanism of action of exogenous agents which modulate intracellular gene function such as pharmaceutical agents and other compounds. Read about the various considerations, approaches and interpretations when conducting a screen to investigate novel compounds and analyzing the screening data.
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In pooled CRISPR drug-gene screens two selection mechanisms can be applied, positive or resistance and negative or sensitivity screening, with each having various considerations and uses
Functional Genomic and Cell Panel Screening can individually provide comprehensive data sets to support therapeutic development, but when utilized in combination they identify novel targets which are cross-validated by independent approaches.
The reasoning and research behind our Dharmacon™ CRISPRi platform recently published in The CRISPR Journal.
Learn about Horizon's CRISPRsc (CRISPR single cell) platform that combines the flexibility of pooled screening with the power of transcriptomics at single cell resolution.
Choosing the right cell-based screen from the plethora of options available can quickly become a complicated decision process. Here, we review two of the major options: cell panel and functional genomic screening.
A revolution is under way in functional genomics which is spearheaded by the CRISPR-Cas9 system and its application to pooled genetic screening. Remarkable new tools, made possible by dCas9, are coming to fruition that will allow for a new kind of interrogation of gene function, allowing us to ask more sophisticated questions about the biology of drug targets.